B.s.c. Tankibayeva N.U.
Karaganda State Medical University , Kazakhstan
OXIDATIVE MODIFICATION OF THE BLOOD PLASMA PROTEINS IN PATIENTS WITH CHRONIC PIELONEPHRITIS AND ASSOCIATED WITH ARTERIAL HYPERTENSION
One of the actual problems now is study of o xidative modification of the blood protein s , as oxidative stress marker in different types of pathologies. Developing of the chronic pielonephritis and arterial hypertension also binds with oxidative metabolism disorders, oxidative stress and blood antioxidant system changes[ 1-8].
The aim of this work was study protein oxidative modification (POM) in blood plasma of patients with chronic pyelonephritis (CP) and chronic pielonephritis associated with arterial hypertension (CP+AH).
We examined 73 patients (age 19 -59 years ) including 42 patients with chronic pyelonephritis and 31 patients with chronic pyelonephritis pielonephritis associated with arterial hypertension . Control group consisted from 25 healthy persons (primary donors).
There were examined in blood plasma the basic and neutral classis of dinitrophenylhydrazons (KDNPG and ADNPG) which are catobolits of protein oxidative modification and defined by R.L. Levine methods (2000).
Shown data demonstrated the same alterations of free-radical oxidations of proteins in blood plasma at patients with chronic pielonephritis and chronic pielonephritis assotiated with arterial hypertension .
Results of the POM study in blood plasma was shown in the table #1.
According to these data KDNPG level of neutral class at patients with chronic pielonephritis decreased in 2 ,3 times than in control group and KDNPG level of basic class decreased in 2,5 times.
More significant alterations of POM data in blood plasma were fixed at patient with CP+AH in compare with control. Thus, the level of KDNPG of neutral class was reliably decreased in 3 ,1 times than in control and the level of KDNPG of basic class was lower in 4 times in compare with control.
The content of the other oxidative products ( ADNPG ) in blood plasma was decreased in 1,8 times for neutral class at patient with CP than control, but the level of basic class of ADNPG in plasma no significant modified compare control.
There were fixed deep oxidative products falling both basic and neutral classis of ADNPG in the next patients group with CP+AH. So, the content of neutral class of ADNPG in blood plasma at patient with CP+AH was lower than control in 2 ,9 times and the content of basic class of ADNPG was lower in 2 times than control.
The POM data (basic and neutral KDNPG ) were reliably lower in 1,4 and 1,6 times at patient with CP+AG in compare with CP. The content of neutral and basic classis of ADNPG in blood plasma at patients with CP were reliably higher in 2,2 times than in patient with CP+AH.
Table1. POM data in blood plasma at patient with CP and CP+AH
|
Data |
Control group |
Patients |
|
|
CP+AH (n=31) |
CP (n=42) |
||
|
KDNPG ( cu / ml ) neutral class , 350 nm |
2,9±0,90 |
0,94±0,16* # |
1.37±0,56* |
|
ADNPG ( cu / ml ) neutral class , 370 nm |
2,7±0,09 |
0,92±0,17* # |
1,40±0,56* |
|
KDNPG(cu/ml) basic class , 430нм |
1.8±0,04 |
0,45±0,09* # |
0,72±0,28* |
|
ADNPG ( cu / ml ) basic class, 530нм |
0,26±0,01 |
0,13±0,04* # |
0,22±0,17 |
|
* - р < 0,05 – compare with control # - р < 0,05 – compare CP+AH and CP |
|||
As a whole, according to our data it was fixed the developing of deep oxidative stress with one-directed modifications of free radical proteins oxidation at patients with chronic pielonephritis and chronic pielonephritis assotiated with arterial hypertension
By our vision, the decrease of POM level was determined by circulation of oxidative proteins in blood plasma which are nonactive to reaction with dinitrophenylhydrazons.
List of reference links:
1.
2. Белоногов Р.Н., Титова Н.М., Дыхно Ю.А. и др. //Сибирский онкологический журнал. – 2009. – № 4 (34) – С.48-53.
3. Stadtman E.R., Levine R.L. Ann N Y Acad Sci. – 2000 ,899 : 191-208.
4. Bertlett B.S., Stadtman E.R. // J Biol Chem . – 1997,272:20313-20316.
5. Дедов И.И. Сахарный диабет: ангиопатии и окислительный стресс: [пособие для врачей] / И.И. Дедов, М.И. Балаболкин, Г.Г. Мамаева . – М.,2003. – 86 с.
6. Pantke U. , Volk T., Schmutzler M. ,Kox W.J. et.al. // Free Radic Biol Med. – 1999 ,27 : 1080- 10866.
7. Молотов-Лучанский В.Б., Кудрявцев С.С., Муравлева Л.Е., Газалиев А.М. //Экспериментальная и клиническая фармакология, 2005. – Т.68, № 5. – С.47-50.
8. Жакипбекова В.А. Окислительный метаболизм у больных гипертонической болезнью на фоне лечения ИАПФ энаприлом / В.А. Жакипбекова, К.А. Алиханова, Л.Е. Муравлева //Медицина и экология, 2004. – № 5 – С.50-52.