Bordunos K., Golodok L., Bondar O.

Oles Honchar Dnipropetrovsk National University


In recent decades, the growth of dysbiotic intestinal diseases have been noted. Changes in the ecological state of the environment, poor nutrition, acute intestinal infections, chronic diseases and dysfunction of the gastrointestinal tract, the widespread use of antibiotics, decreased immune reactivity may be the causes of disturbed equilibrium between the resident microflora and of intestinal dysbiosis. The need to monitor the intestine microbicenosis of infants and children during the first years of life is of particular importance, as initially formed microbial set, determines microbiological status a child throughout a life. At significant deviations of the normal flora of the body it is necessary to correcti it by introducing products based on microbial cultures – probiotics.

The research on creating more sophisticated and active drugs on the basis of various groups of obligate microflora microorganisms are conducted worldwide. The mportant role in these experiments is occupied by Bacillus bacteria, forming the basis of many well-known probiotics (Biosporin, baktosubtilin, subalin, enterohermin, biosubtilin).

These microorganisms, due to high adaptive capacity, are widely spread in nature, especially in those objects with which people contact as closely as possible (air, water, food, etc.). Therefore bacilli enter the human body continuously and in large quantities, preserving activity throughout the gastrointestinal tract. Furthermore, Bacillus bacteria are an important component of the exogenous microflora and areconsidered to be the most effective to create biological products.

One of the conditions of any strain of bacteria of the genus Bacillus to create a biological product is its antagonistic action against the conditionally and pathogenic and pathogenic microorganisms, and also display of different types of activity, which resulted into the aim of work.

Making preparations for the correction of dysbiotic violations is very important nowadays. We have identified strains of Bacillus bacteria, investigated the enzymatic activity of these strains and have shown that they exhibit antagonistic, cellulolytic, xylanaselytic activies and some of them can show pektynase and polihalakturonase activity. Out of 12 identified strains of aerobic spore-forming bacilli – 83.3% showed antagonistic activity to C. albicans, and 75% showed activity to S. aureus. To S. flexnery antagonistic activity has not been identified. All strains of the investigated bacilli are producers of cellulase, 80% are capable of xylanaselytic activity and only in some strains polihalakturonase and pectin esterase have been. This indicates that the identifed strains can be used as probiotic preparations for correction of dysbiotic disorders of the human gastrointestinal tract.