Tonkonog A. A., Shtemenko N. I., Osadcha O. V.
Oles Honchar Dnipropetrovsk National University
THE INFLUENCE OF CISPLATIN AND ANTITUMOR RHENIUM-PLATINUM SYSTEM ON BIOCHEMICAL AND MORPHOLOGICAL CHARACTERISTICS OF TUMOR-BEARING RATS’ BLOOD
The study of red blood cells in the pathogenesis of the tumor growth, the emergence and the mechanism of developing anemia, which is a dangerous complication of malignant tumors, is an important direction of Biochemistry carcinogenesis. The search of new drugs that can correct the anemic state in condition of the tumor development has both theoretical and practical importance.
The given research is devoted to the problem of finding new ways of treating Guerin carcinoma. It showed that the introduction of cisplatin led to the significant damage of RBC state and increased the quantity of pathological forms of RBC. Introduction of the rhenium compounds as a part of the Re-Pt system had antianaemic properties, influenced positively on morphological and biochemical parameters of blood.
The experiments conducted by our group testified that the compounds of rhenium, administered separately or as a part of antitumor Rhenium-Platinum system, had the antioxidant effect on the system of red blood of tumor-bearing rats, which was the basis for further research of the properties of these compounds.
The aim of this study was to investigate the biochemical and morphological picture of the tumor-bearing rats’ blood under administration of cisplatin, antitumor Rhenium-Platinum (Re-Pt) systems and their components. The blood parameters were investigated: hemoglobin, hematocrit, total number of erythrocytes and pathological forms of erythrocytes.
The study shows that the development of Guerin’s carcinoma leads to the reduction of hemoglobin concentration (33 %), hematocrit level (6.1 %), the decrease in the general among of erythrocytes (32.8 %) and the increase in the level of pathological erythrocytes forms (on average 6 times) compared to the control group, which allowed to identify the anemic state of the experimental animals. Under administration of cisplatin there was the reduction of the level of hematocrit (25.3 %), the general among of erythrocytes (10.6 %) and the increase in erythrocytes pathological forms (2.1 times) in comparison with the group of tumor-bearing rats, that evidenced about a further deepening of the anemic state of the experimental animals. It is shown that under administration of cluster compounds of rhenium (regardless of route of administration), the number of red blood cells increased (on average by 32 %), the pathological forms of erythrocytes decreased (on average by 75 %), the hematocrit level became normal and hemoglobin increased (by 40–48 %) in comparison with the group of tumor-bearing rats, which testified the antianemic properties of rhenium compounds. It was found out that regardless of the anti-cancer efficacy, rhenium cluster compounds influence the animal red blood state in models of carcinogenesis and may be proposed as modulators of toxic act of cisplatin and other hemolytics.
So, the normalization and restoration of biochemical and morphological characteristics of the blood of rats with Guerin carcinoma proved the ability of the rhenium compounds and antitumor Re-Pt systems to manifest their antianemic properties.